With suggestions that limiting sedentary time can prevent obesity, the need to identify a cut-off point for daily sedentary time is being recognized. Therefore, this study aims to examine the dose-response relationship between daily sedentary time and obesity and to suggest a cutoff of daily sedentary time for the prevention of obesity. Data from the 2003 to 2006 National Health and Nutrition Examination Survey (NHANES) were analyzed. A total of 5,127 adults (>19 years old) were included in the present study.